Characterization of a yeast mitochondrial promoter by deletion mutagenesis.

Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis.

Cyclic adenosine monophosphate (cAMP) is an important modulator of platelet responses to agonists. Cyclic nucleotide phosphodiesterase (PDE) controls intracellular cAMP concentrations by hydrolyzing it to AMP. The major PDE activity in platelets is PDE3A (cyclic guanosine monophosphate [cGMP]-inhibited PDE). To obtain structural information on platelet PDE3A, we cloned the enzyme cDNA from a hu...

Characterization of mitochondrial ribosomes from yeast.

A method for the preparation of mitochondrial ribosomes from yeast is presented. Mitochondrial ribosomes differ from their cytoplasmic counterpart in nucleotide composition, electrophoretic mobility of a number of their respective ribosomal proteins, and thermal denaturation profiles. The mitochondrial and cytoplasmic ribosomes have identical sedimentation coefficients (80 S) and dissociate int...

A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

Functional Characterization of TaSnRK2.8 Promoter in Response to Abiotic Stresses by Deletion Analysis in Transgenic Arabidopsis

Drought, salinity, and cold are the major factors limiting wheat quality and productivity; it is thus highly desirable to characterize the abiotic-stress-inducible promoters suitable for the genetic improvement of plant resistance. The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family genes show distinct regulatory properties in response to abiotic stresses. The present study cha...

Characterization of a DNA binding domain in the C-terminus of HIV-1 integrase by deletion mutagenesis.

The integrase (IN) protein of human immunodeficiency virus type 1 (HIV-1) catalyzes site-specific cleavage of 2 bases from the viral long terminal repeat (LTR) sequence yet it binds DNA with little DNA sequence specificity. We have previously demonstrated that the C-terminal half of IN (amino acids 154-288) possesses a DNA binding domain. In order to further characterize this region, a series o...